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現在位置首頁>實驗試劑制品>干細胞>凍存液> Biolife 210102 CryoStor CS10 Freeze Media細胞凍存液

商品編號:73192
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Biolife 210102 CryoStor CS10 Freeze Media細胞凍存液

價    格:詢價

產    地:美國更新時間:2019/7/15 13:41:34

品    牌:BioLife Solutions型    號:210102

狀    態:正常點擊量:578

18253167905
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山東博科干細胞應用研究院有限公司

聯 系 人:母經理

電     話:18253167905

傳     真:

等     級: (第 6年)

性     質:生產型,貿易型,

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CryoStor?是Biolife公司特別優化的細胞凍存液,在極低溫度 (-70oC to -196oC)下準備和保存細胞。預混DMSO,為細胞和組織的冷凍、儲存和解凍過程提供安全的保護環境。CryoStor?通過調控凍存過程的分子生物學反應,無血清、蛋白或具有高細胞毒性的試劑,就能增強細胞活力和功能。

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CryoStor CS10細胞凍存液特點

用于細胞和組織的高級生物保存液:

預混DMSO

無血清、無蛋白

USP級高質量成分、cGMP級生產、FDA master文件

滅菌、內毒素和細胞功能檢測

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DMSO無血清細胞凍存液訂購信息

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品牌

貨號

產品描述

規格

BioLife

210210

CryoStor CS10 Freeze Media 細胞凍存液

1000mL/袋

BioLife

210202

CryoStor CS10 Freeze Media 細胞凍存液

100mL/袋

BioLife

210102

CryoStor CS10 Freeze Media 細胞凍存液

100mL /瓶

BioLife

210374

CryoStor CS10 Freeze Media 細胞凍存液

16mL /瓶

BioLife

210373

CryoStor CS10 Freeze Media 細胞凍存液

10mL /瓶

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DMSO無血清細胞凍存液適用細胞

多種類型細胞,包括如肝細胞之類的敏感細胞

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CryoStor CS10 Freeze Media 使用與凍存方法

CryoStor? Usage and Cryopreservation Protocol

細胞準備

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor? solution.

加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor?

a. Cell concentrations: 0.5-10 x 106

?cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor? - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

儲存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

細胞復蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.

Passive thaw is not recommended.

9) Dilute cell/CryoStor? mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue?) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.






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