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現在位置首頁>實驗試劑制品>干細胞>凍存液> BioLife CryoStor CS5 細胞DMSO凍存液 貨號205102

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BioLife CryoStor CS5 細胞DMSO凍存液 貨號205102

價    格:詢價

產    地:美國更新時間:2019/7/15 13:41:34

品    牌:BioLife Solutions型    號:205102

狀    態:正常點擊量:454

18253167905
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山東博科干細胞應用研究院有限公司

聯 系 人:母經理

電     話:18253167905

傳     真:

等     級: (第 6年)

性     質:生產型,貿易型,

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BioLife Solutions GMP級CryoStor CS5 Freeze Media細胞DMSO凍存液

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產品英文名?BioLife Solutions CryoStor CS5 Freeze Media

產品中文名?BioLife Solutions CryoStor CS5細胞DMSO凍存液

產品介紹??BioLife Solutions CryoStor?是BioLife公司特別優化的細胞凍存液,在極低溫度 (-70oC to -196oC)下準備和保存細胞。預混DMSO,為細胞和組織的冷凍、儲存和解凍過程提供安全的保護環境。CryoStor?通過調控凍存過程的分子生物學反應,無血清、蛋白或具有高細胞毒性的試劑,就能增強細胞活力和功能。

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產品訂購信息

品牌

貨號

產品描述

包裝

BioLife Solutions

205373

CryoStor CS5?Freeze Media 細胞DMSO凍存液

5×10mL

BioLife Solutions

205102

CryoStor CS5?Freeze Media 細胞DMSO凍存液

100 mL/

BioLife Solutions

205202

CryoStor CS5?Freeze Media 細胞DMSO凍存液

100 mL/

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CryoStor CS5細胞凍存液特點

用于細胞和組織的高級生物保存液

預混DMSO

無血清、無蛋白

USP級高質量成分、cGMP級生產、FDA master文件

滅菌、內毒素和細胞功能檢測

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DMSO無血清細胞凍存液適用細胞

多種類型細胞,包括如肝細胞之類的敏感細胞

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BioLife Solutions CryoStor CS5?細胞DMSO凍存液cGMP級生產

MATERIALS ARE MANUFACTURED UNDER cGMP

636511090214651483525.jpg?

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CryoStor? Usage and Cryopreservation Protocol使用與凍存方法

細胞準備

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor? solution.

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加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor?

a. Cell concentrations: 0.5-10 x 106cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor? - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

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儲存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

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細胞復蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.Passive thaw is not recommended.

9) Dilute cell/CryoStor? mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

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Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

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*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue?) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

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BioLife Solutions?CryoStor CS5/CS10?Freeze Media?細胞凍存液

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品牌

貨號

產品描述

包裝

BioLife Solutions

210102

CryoStor CS10 Freeze Media 細胞DMSO凍存液

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100 mL/瓶

BioLife Solutions

210210

CryoStor CS10 Freeze Media 細胞DMSO凍存液

1000 mL/袋

BioLife Solutions

210202

CryoStor CS10 Freeze Media 細胞DMSO凍存液

100 mL/袋

BioLife Solutions

210374

CryoStor CS10 Freeze Media 細胞DMSO凍存液

16 mL/瓶

BioLife Solutions

210373

CryoStor CS10 Freeze Media 細胞DMSO凍存液

10 mL/瓶

BioLife Solutions

205373

CryoStor CS5?Freeze Media 細胞DMSO凍存液

5×10mL

BioLife Solutions

205102

CryoStor CS5?Freeze Media 細胞DMSO凍存液

100 mL/

BioLife Solutions

205202

CryoStor CS5?Freeze Media 細胞DMSO凍存液

100 mL/

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CS5和CS10主要區別是規格包裝不同。






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