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pll3.7 慢病毒系统
pll3.7 慢病毒系统
  • pll3.7 慢病毒系统

pll3.7 慢病毒系统

产品报价:询价

更新时间:2012/2/8 16:52:40

地:上海

牌:

号: 10ug

厂商性质: 生产型,贸易型,服务型,

公司名称: 上海希匹吉生物技术有限公司

产品关键词:

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Plasmid: pLL3.7

Vendor:

Addgene Plasmid Repository

Catalog Number:

Addgene Plasmid 11795

Gene/insert name:

none

Insert size (bp):

Unknown

Type of vector:

Mammalian xpression,Lentiviral,RNAi,Cre/Lox

Backbone size (bp):

7650

Cloning site 5':

HpaI

Site destroyed during cloning:

No

Cloning site 3':

XhoI

Site destroyed during cloning:

No

5' Sequencing primer:

U6 (5'-cagtgcaggggaaagaatagtaga c-3') 

Bacteria resistance:

Ampicillin

High or low copy:

High Copy

Grow in standard E. coli @ 37C:

Yes

Please specify bacterial strain for growth and growth condition:

Although this plasmid grows in standard bacteria, to reduce the chance of recombination we recommend that you grow the plasmid at 30oC in Stbl3 cells from Invitrogen.

Selectable markers:

GFP

Sequence:

View Sequence

Author's Map:

View Map

Plasmid Provided In:

DH5a

 

LOCUS       pLL3.7 7650 bp DNA SYN 23-Aug-2007
DEFINITION  pLL3.7
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="pLL3.7"
                     /mol_type="other DNA"
     misc_feature    318..605
                     /label="CAG_enhancer"
     promoter        239..815
                     /label="CMV_immearly_promoter"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
     misc_feature    835..1015
                     /label="truncHIV-1_3_LTR"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     misc_feature    1686..1919
                     /label="RRE"
     CDS             1564..2451
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"
     misc_feature    2450..2465
                     /label="cPPT"
     misc_feature    2951..2967
                     /label="pBluescriptKS_primer"
     misc_feature    3010..3043
                     /label="loxP"
     misc_feature    3163..3450
                     /label="CAG_enhancer"
     promoter        3108..3660
                     /label="CMV_immearly_promoter"
     misc_feature    3617..3637
                     /label="CMV_fwd_primer"
     promoter        3618..3687
                     /label="CMV_promoter"
     misc_feature    complement(3756..3777)
                     /label="EGFP_N_primer"
     gene            3711..4427
                     /label="EGFP"
                     /gene="EGFP"
     CDS             3711..4430
                     /label="EGFP"
                     /gene="EGFP"
                     /note="ORF frame 3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     CDS             complement(3696..4466)
                     /label="ORF frame 3"
                     /translation="MLYEVIRSLDEFLLVQLVHAESDPGGGHELQQDHVIALLVGVFA
                     QGGLGAQVVVVGQQHGAVADGGVLLVVVGELHAAVLDVVADLEVHLDAVLLLVGHDID
                     VVAVVVVLQLVPQDVAVLLEVDALQLDAVHQGVALELHLGAGLVVAVVLEEDGALLDV
                     AFGHGGLEEVVLLHVVGVAAEALHAVGQGGHEGGPGHGQLAGGADELQGQLAVGGIAL
                     ALAGHAELVAVYVAVQLDQDGHHPGEQLLALAHHGGDR*"
     misc_feature    4364..4385
                     /label="EGFP_C_primer"
     misc_feature    4450..4483
                     /label="loxP"
     misc_feature    4541..5128
                     /label="WPRE"
     misc_feature    complement(5131..5147)
                     /label="pBluescriptKS_primer"
     CDS             4642..5682
                     /label="ORF frame 1"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA
                     AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW
                     CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH
                     PDCTGSLWLDQI*"
     misc_feature    5319..5334
                     /label="cPPT"
     misc_feature    5319..5340
                     /label="U3PPT"
     misc_feature    5656..5836
                     /label="HIV-1_5_LTR"
     misc_feature    5656..5836
                     /label="truncHIV-1_3_LTR"
     rep_origin      complement(5883..6502)
                     /label="pBR322_origin"
     CDS             complement(6657..7517)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            complement(6657..7517)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     promoter        complement(7559..7587)
                     /label="AmpR_promoter"
ORIGIN
    1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
   61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
  121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
  181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
  241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
  301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
  361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
  421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
  481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
  541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
  601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
  661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
  721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
  781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
  841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
  901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
  961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
 1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
 1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
 1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
 1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
 1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
 1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
 1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
 1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
 1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
 2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
 2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
 2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
 2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
 2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
 2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
 2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
 2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
 2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
 2581 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg ccgccatctc
 2641 taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc
 2701 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac
 2761 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa
 2821 gagatacaaa tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact
 2881 gtaaagtaat tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgttaacgc
 2941 gcggtgaccc tcgaggtcga cggtatcgat aagctcgctt cacgagattc cagcaggtcg
 3001 agggacctaa taacttcgta tagcatacat tatacgaagt tatattaagg gttccaagct
 3061 taagcggccg cgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt
 3121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
 3181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
 3241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
 3301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
 3361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
 3421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
 3481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
 3541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
 3601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
 3661 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca
 3721 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
 3781 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
 3841 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
 3901 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
 3961 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
 4021 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
 4081 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
 4141 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
 4201 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
 4261 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
 4321 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
 4381 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtag gaattcgtcg
 4441 agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc
 4501 ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
 4561 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
 4621 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
 4681 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
 4741 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
 4801 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
 4861 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
 4921 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
 4981 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
 5041 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
 5101 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa
 5161 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
 5221 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
 5281 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
 5341 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
 5401 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
 5461 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
 5521 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
 5581 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
 5641 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
 5701 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
 5761 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
 5821 tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
 5881 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
 5941 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
 6001 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
 6061 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
 6121 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
 6181 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
 6241 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
 6301 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
 6361 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
 6421 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
 6481 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
 6541 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
 6601 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
 6661 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
 6721 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
 6781 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
 6841 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
 6901 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
 6961 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
 7021 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
 7081 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
 7141 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
 7201 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
 7261 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
 7321 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
 7381 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
 7441 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
 7501 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
 7561 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
 7621 cgcacatttc cccgaaaagt gccacctgac

 

 

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